Yohei Morishita,† Huiping Zhang,‡ Tohru Taniguchi,§ Keiji Mori,# and Teigo Asai*,†
Heterologous expression of a unique biosynthetic gene cluster comprising a highly reducing polyketide synthase and a stand alone thioesterase genes in Aspergillus oryzae enabled us to isolate a novel 34-membered polyene macrolide, phaeospelide A (1). This is the first isolation of a fungal polyene macrolide and the first demonstration of fungal aliphatic macrolide biosynthetic machinery. In addition, sequence similarity network analysis demonstrated the existence of a large number of BGCs for novel fungal macrolides.
